Fig. 4
The nature of the edits are similar across cell lines, transfection conditions and editing efficiencies. A While the percentage of sequences showing some form of indel is markedly higher in cells transfected under Lo/Matpos conditions, the proportion and types of indels that occur are similar across the board (n = 3 per condition – 1× 18M, 1× 32F, 1× 42F). B The single nucleotide most frequently inserted follow a similar pattern between cell lines and conditions for each target. There is greater variability in the Matrigel negative conditions due to the lower levels of editing (n = 3 per condition – 1× 18M, 1× 32F, 1× 42F)