Fig. 5From: Improved CRISPR/Cas9 gene editing in primary human myoblasts using low confluency cultures on MatrigelExpression of MYF5 and MYOD1 are both decreased in MYF5 edited cells. Levels of MYF5 and MYOD1 in MYF5-edited cells (MYF5-KO) are shown relative to the average expression of the negative controls (NC). There is a clear decrease in expression of both genes when compared to cells transfected with the scrambled NC guide (n = 3 per condition – 1× 18M, 1× 32F, 1× 42F). Error bars show mean ± SD. ** = p < 0.01Back to article page