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Fig. 4 | Skeletal Muscle

Fig. 4

From: Six1 promotes skeletal muscle thyroid hormone response through regulation of the MCT10 transporter

Fig. 4

Six1-binding profile and epigenetic marks at the genes coding the two main thyroid hormone transporters. At each locus, the gene name, genomic position (mm9 coordinates), and length of the interval shown are given. The Six1 ChIP-seq signal represents data from primary proliferating myoblasts or differentiated myotubes, quantitated in sliding windows across the genome in reads per million sequenced, and subtracted from the signal in input chromatin control sample. The ATAC-seq data in quadriceps and soleus is shown for inferred nucleosome-free regions (insert sites between 10 and 130 base pairs) and quantitated in bins of 10 base pairs in counts per millions sequenced (CPM). The H3K4me2, indicative of enhancers and promoters, and H3K27ac, indicative of active promoters and enhancers, are quantitated in bins of 10 and CPM. A Signal at the Slc16a10 (MCT10) locus. The pink box upstream of the Slc16a10 start site shows the location of a putative Six1-bound enhancer, active predominantly in fast-twitch quadriceps, compared to slow-twitch soleus. B Magnification of the −48 kb enhancer at the Slc16a10 locus. C Signal at the Slc16a2 locus. D Genomic signal at the locus comprising the Myh2 to Myh8 genes. E Genomic signal at the Myh7 locus. F Signal at the Myogenin gene. The blue box represents the area amplified by PCR, in Fig. 5

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