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Fig. 6 | Skeletal Muscle

Fig. 6

From: Six1 promotes skeletal muscle thyroid hormone response through regulation of the MCT10 transporter

Fig. 6

Six1 is needed for MCT10 expression and activity of the TH pathway. A Western blots assaying Six1 protein expression levels under siRNA knockdown condition. siNS represents a control, non-silencing RNA duplex. Note that animals 1 to 4 and 5 to 7 were processed on different days and the western blots were done on different gels and membranes. B Densitometric quantitation of Six1 abundance shown in panel A. Error bars indicate the SEM. The difference in Six1 protein abundance is statistically significant by a one-tailed paired T test relative to siNS (*p value < 0.05). The experiment was performed with seven mice treated identically. C mRNA expression levels of Six1 and MCT10 in electroporated mouse TA muscle after Six1 knockdown, quantified by qRT-PCR. Six4 and MCT8 are shown as members of the same gene families with invariant expression in this experiment. mRNA levels were normalized to the geometric mean of those of 18S rRNA and Actnb. Data represent an average of 7 biological replicates (animals). The normalized expression data is given, with each individual replicate (mouse) shown in a distinct color. Error bars indicate the mean ± SEM of the replicates. P values in red indicate statistically significant decreases in levels of expression as determined by a one-tailed paired Student’s T test relative to siNS (p value < 0.05). D mRNA expression level of a panel of four TH pathway target genes in the control and Six1 knockdown samples from panel C. Two-way ANOVA (testing mRNA levels as a function of which gene was tested and which siRNA was received) indicates significant reduction of the TH signaling gene panel with Six1 knockdown (p value = 0.001)

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