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Fig. 1 | Skeletal Muscle

Fig. 1

From: ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index

Fig. 1

CAPZB depletion results in a trend of less cell viability and differentiation. CAPZB was depleted in myotubes using two different si-RNAs (#1 and #2), and a negative control si-RNA was used (si-ctrl). A, B Western blot assays were performed (A) and quantified by densitometry (B). C Immunofluorescence experiments were performed in myotubes after 4 days of differentiation. Cells were stained with DAPI (cyan) and an antibody recognizing the myosin heavy chain protein (MYH) (magenta). Scale bar = 200 Îĵm. D The number of nuclei per unit area (mm2) was determined manually. E Muscle cell differentiation was determined by calculating the fusion index manually. Results are shown as mean + s.e.m., *p < 0.05, Welch’s t test, n = 3 independent experiments

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