Fig. 2
From: ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index
ViaFuse macro implementation steps. A The first image in cyan was the original DAPI image before any analyses. The second image was generated after thresholding to make the image binary. The third image was generated after the application of a median filter and the filling of holes. The final image was generated by applying a watershed step. B The first image in cyan was the original DAPI image before any analyses. The same steps described in A were taken to achieve the next DAPI image. The MYH image (original) in magenta underwent the same analyses as the DAPI image. The final images were subtracted from one another resulting in a composite of DAPI and MYH after subtraction