Fig. 5
From: ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index
ViaFuse overcomes limitations of MyoCount. A Images demonstrating how ViaFuse-V can more accurately determine nuclear clumps compared to MyoCount by (a) applying a watershed step (light blue rectangle) and (b) estimating the number of nuclei in particles greater than 251âÎĵm2 by dividing their sizes by the mode of the nuclei sized 50â250âÎĵm2. B ViaFuse-F distinguished myoblasts that are close to myotubes. C Images demonstrating that ViaFuse-F defined myotubes borders accurately in situations where MyoCount considered them incompletely