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Table 1 Analysis of the total number of nuclei analysis using ViaFuse-V: parameter and default settings. The purpose of each parameter and its function is explained in the description column and the default setting of that parameter is shown

From: ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index

Parameter

Default setting

Description

Multiplication

1

Multiplies the image by the specified value. The default value of 1 indicates that there is no multiplication. Value must be greater than 1 to increase the brightness of the image. Useful when image is too dark. User is prompted to change this setting if desired

Median filter

1 pixel

Reduces noise in the image by replacing each pixel with the median of the neighboring pixel values. User is not prompted to change median radius value but may do so in the macro script

Analyze particles step 1

50–250 Îĵm2

Analyzes DAPI image for nuclei of these areas to obtain number of nuclei. User is not prompted to change this setting but may do so in the macro script

Analyze particles step 2

251 Îĵm2–infinity

Analyzes DAPI image for nuclei of these areas to obtain the areas of nuclei found in clumps. User is not prompted to change this setting but may do so in the macro script