From: ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index
Parameter | Default setting | Description |
---|---|---|
Multiplication | 1 | Multiplies each of the images by the specified value. The default value of 1 indicates that there is no multiplication. Value must be greater than 1 in order to increase the brightness of the image. Useful when image is too dark. User is prompted to change this setting if desired |
Median filter | 1 pixel | Reduces noise in each of the images by replacing each pixel with the median of the neighboring pixel values. User is not prompted to change median radius value but may do so in the macro script |
Gaussian blur (sigma) | 2 pixels | Smooths MYH image by giving a higher weight to edge pixels than pixels inside the image, making the myotube outlines clearer. User is not prompted to change the sigma value but may do so in the macro script |
Analyze particles step 1 | 50â250âÎĵm2 | Analyzes DAPI image for nuclei of these areas to obtain number of nuclei. User is not prompted to change this setting but may do so in the macro script |
Analyze particles step 2 | 251âÎĵm2âinfinity | Analyzes DAPI image for nuclei of these areas to obtain the areas of nuclei found in clumps. User is not prompted to change this setting but may do so in the macro script |