Fig. 4

Representative images (200fold magnification, scale bar = 50 μm) of soleus muscle cross sections stained for BTX of WT-NOX (A) and WT-CIH (B) are shown. Postsynaptic NMJ area in soleus muscle (C). Postsynaptic NMJ area normalized to myofiber CSA (D) and NMJ length relative to myofiber perimeter (E) as well as percentage of fragmented NMJ in soleus muscle (F). Representative images (400-fold magnification) of BTX-stained AChR distribution at muscular NMJ in soleus muscle (G, H). G Postsynaptic BTX-stained NMJ from WT-NOX mouse (white arrow). H Fragmented postsynaptic BTX-stained NMJ of WT-CIH mouse is (black arrow). Values are given as mean + SEM; n = 8 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001, significance between CIH and NOX; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, significance between WT and iNOS^−/−