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Fig. 6 | Skeletal Muscle

Fig. 6

From: Effect of chronic intermittent hypoxia (CIH) on neuromuscular junctions and mitochondria in slow- and fast-twitch skeletal muscles of mice—the role of iNOS

Fig. 6

Representative images (200fold magnification, scale bar = 50 μm) of soleus muscle cross sections stained for CD68 of WT-NOX (A), WT-CIH (B), iNOS−/−-NOX (C), and iNOS−/−-CIH (D) are shown. CD68-positive cells are marked with arrows. Density of IL 1β- (E) and CD68- (F) positive cells in the soleus muscle is shown. Mean number of macrophages per NMJ (G) and mean number of macrophages per denervated NMJ (H). Merge of confocal projection images of representative NMJ from the soleus muscle stained for nerve terminal vAChT (green) and CD68 (red) showing the colocalization of NMJ and macrophages (H). Values are given as mean + SEM, n = 8 animals per group. * p < 0.05, significance between CIH and NOX, #p < 0.05, significance between WT and iNOS−/−

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