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Fig. 5 | Skeletal Muscle

Fig. 5

From: Endurance exercise attenuates juvenile irradiation-induced skeletal muscle functional decline and mitochondrial stress

Fig. 5

Nitrosative and mitochondrial oxidative stress in irradiated muscle is reduced with exercise. a Soleus whole muscle protein lysate oxyblot from non-irradiated (Rad CL) and irradiated (Rad RL) muscle in sedentary and exercised mice. b Quantification of oxidized proteins from a normalized to ponceau stain and non-irradiated (Rad CL) sedentary muscle. N = 4–5 mice per condition. c Soleus whole muscle protein lysate 3-nitrotyrosine (3-NT) immunoblot from non-irradiated (Rad CL) and irradiated (Rad RL) muscle in sedentary and exercised mice. d Quantification of 3-NT levels normalized to GAPDH and non-irradiated sedentary controls from c immunoblot. N = 4–5 mice per condition. e Images of MitoSOX Red-loaded FDB myofibers from non-irradiated (Rad CL) and irradiated (Rad RL) muscle in sedentary and exercised mice at time 0 and time 10 (min). f Quantification of MitoSOX fluorescence from e. Technical n is displayed directly on the graph, biological n = 5 mice per condition. Two-way ANOVA with multiple comparisons. */#p < 0.05, **p < 0.01. Isolated asterisks denote the ANOVA group effect of exercise. Isolated pound signs denote the ANOVA group effect of radiation. Data displayed as mean ± s.e.m

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