Fig. 6

Exercise adapts the mitochondrial gene expression profile of irradiated muscle. a Whole muscle mRNA expression from 3-month-old WT mouse irradiated (Rad RL) and non-irradiated (Rad CL) muscle after 1 month of VWR exercise (running) or sedentary control for Dnm1l, bFis1, cMfn1, and dPgc1α. mRNA levels are reported as fold change relative to Gapdh and normalized to sedentary non-irradiated control. N = 3 mice for each condition. e TA whole muscle protein lysate immunoblot for PGC1α, MFN1, and VDAC. Quantification of f MFN1, g PGC1a, and h VDAC protein levels, as displayed in the immunoblot from TA lysate I, normalized to GAPDH and non-irradiated sedentary controls. n = 3 mice per condition except PGC1α and VDAC, n = 6. Two-way ANOVA with multiple comparisons. *^/#p < 0.05, **^/##p < 0.01, ***p < 0.001, ****p < 0.0001. Isolated asterisks denote the ANOVA group effect of exercise. Isolated pound signs denote the ANOVA group effect of radiation. A significant interaction between variables was observed in the analysis of Pgc1α transcript levels in d, p = 0.0113. Data displayed as mean ± s.e.m