Fig. 6

STAT3 inhibition prevents muscle atrophy in the MCT rat. A SD rats were treated with MCT or saline on day 1, with or without intraperitoneal injection of Stattic daily for 2 weeks since day 14. The effects of Stattic on the main features of PAH model were examined, including B RVSP, C body weight, and D gastrocnemius muscles mass. E Cross-sections cut from the gastrocnemius muscle and stained with wheat germ agglutinin (blue). Scale bar is 25μm, n=3. F The cross-sectional areas of approximately 250 myofibers per group were determined and the distribution of myofiber cross-sectional area. Data are shown as mean ± SEM. Versus vehicle control, *P < 0.05, **P < 0.01, ***P < 0.001; versus MCT control, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001; n=8 rats