Fig. 5
From: Alternative splicing diversifies the skeletal muscle transcriptome during prolonged spaceflight
DAS of ubiquitin-proteasome pathway genes during spaceflight. A Bars and dashed lines represent exons and introns, respectively, of Ubap1 pre-mRNA, with exon numbering below. Untranslated regions (UTR) are denoted in black. Solid lines connecting exons 4 and 5 to nearby exons represent mutually exclusive splicing. The UBA1 domain encoded by exon 5 is depicted in green. B The exon 4-excluded Ubap1 splice isoform possesses the UBA1 domain-encoding region. The exon 4-included Ubap1 splice isoform does not possess the UBA1 domain-encoding region. C Box-and-whisker plots depict the distribution (mean value, upper/lower quartiles, and maximum/minimum) of PSI (percent spliced in of a specific exon) values for exon 4 (first exon of mutually exclusive exon 4/5 event on + strand) in ground and flight for gastrocnemius (left pair) and quadriceps (right pair). △PSI (change in percent spliced in of a specific exon) values between ground and flight are provided in numerical form. Asterisks represent significance as reported by rMATS-turbo (nsFDR > 0.05, *FDR < 0.05). D Bars and dashed lines represent exons and introns, respectively, of Usp14 pre-mRNA, with exon numbering below. Untranslated regions (UTR) are denoted in black. Solid lines connecting exons 3 and 4 to nearby exons represent mutually exclusive splicing. The UBL domain encoded by exon 4 is depicted in yellow. E The exon 4-included Usp14 splice isoform possesses the UBL domain-encoding region. The exon 4-excluded Usp14 splice isoform does not possess the UBL domain-encoding region. F Box-and-whisker plots depict the distribution (mean value, upper/lower quartiles, and maximum/minimum) of PSI (percent spliced in of a specific exon) values for exon 4 (first exon of mutually exclusive 3/4 event on − strand) in ground and flight for gastrocnemius (left pair) and quadriceps (right pair). △PSI (change in percent spliced in of a specific exon) values between ground and flight are provided in numerical form. Asterisks represent significance as reported by rMATS-turbo (nsFDR > 0.05, *FDR < 0.05)