Fig. 8
From: Alternative splicing diversifies the skeletal muscle transcriptome during prolonged spaceflight
DAS of musculoskeletal splicing regulators during spaceflight. A Bars and dashed lines represent exons and introns, respectively, of Mbnl1 pre-mRNA with exon numbering below. Untranslated regions (UTR) are denoted in black. Solid lines connecting exon 2 to nearby exons represent exon skipping. Two encoded start codons are depicted in green. Four encoded zinc-finger domains (ZF1-4) are depicted in yellow. B The exon 2-included Mbnl1 splice isoform possesses ZF1, 2, 3, and 4-encoding regions. The exon 2-excluded Mbnl1 splice isoform possesses only ZF3 and 4-encoding regions. C Box-and-whisker plots depict the distribution (mean value, upper/lower quartiles, and maximum/minimum) of PSI (percent spliced in of a specific exon) values for exon 2 in ground and flight for gastrocnemius (left pair) and quadriceps (right pair). â–³PSI (change in percent spliced in of a specific exon) values between ground and flight are provided in numerical form. Asterisks represent significance as reported by rMATS-turbo (nsFDR > 0.05, *FDR < 0.05, **FDR < 0.01, ***FDR < 0.001). D Table depicts genes with known Mbnl1 binding motifs. E Bars and dashed lines represent exons and introns, respectively, of Rbfox1 pre-mRNA with exon numbering below. Untranslated regions (UTR) are denoted in black. Solid lines connecting exons B40 (light blue) and exon M43 (red) to nearby exons represent mutually exclusive splicing. F The brain-specific Rbfox1 splice isoform has exon B40 included and exon M43 excluded, while the muscle-specific Rbfox1 splice isoform has exon B40 excluded and exon M43 included. G Box-and-whisker plots depict the distribution (mean value, upper/lower quartiles, and maximum/minimum) of PSI (percent spliced in of a specific exon) values for exon B40 in ground and flight for gastrocnemius (left pairs) and quadriceps (right pairs). â–³PSI (change in percent spliced in of a specific exon) values between ground and flight are provided in numerical form. Asterisks represent significance as reported by rMATS-turbo (nsFDR > 0.05, *FDR < 0.05, **FDR < 0.01, ***FDR < 0.001). H Table depicts genes with known Rbfox1-binding motifs