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Fig. 2 | Skeletal Muscle

Fig. 2

From: Absence of the Z-disc protein α-actinin-3 impairs the mechanical stability of Actn3KO mouse fast-twitch muscle fibres without altering their contractile properties or twitch kinetics

Fig. 2

Unloaded speed of shortening during external field stimulation of single enzymatically dissociated intact FDB fibres. a Example images during a shortening sequence of a Actn3KO single fibre stimulated at 100 Hz for 250 ms and recorded at 4166 fps. Also shown (right panel, fibre image on a black background) are the automated analysis images from which the shortening parameters, fibre length and shortening velocity were recorded. b Analysed time traces of these parameters for the same fibre at indicated stimulation frequencies. c Minimum shortening length was not different between WT and Actn3KO mice; however, there was a significant reduction in both genotypes at higher frequencies 20–100 Hz as indicated *. d Absolute maximum shortening velocities were not different between genotypes apart from 30 Hz where Actn3KO were significantly faster as indicated by *. Significance WT vs. KO based on one-way ANOVA test indicated as follows: *p < 0.05

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Skeletal Muscle

ISSN: 2044-5040

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