Skip to main content
Fig. 7 | Skeletal Muscle

Fig. 7

From: Absence of the Z-disc protein α-actinin-3 impairs the mechanical stability of Actn3KO mouse fast-twitch muscle fibres without altering their contractile properties or twitch kinetics

Fig. 7

Second harmonic imaging and quantitative morphometry imaging in single dissociated EDL fibres reveal misregistered myofibrillar ultrastructure in Actn3KO. a Representative example images from the middle plane of a single WT and Actn3KO EDL fibre (top) with the automatically detected verniers shown as red dots and short red lines the VD and CAS values derived from the morphometry analysis are given in text on the bottom right of each panel. A magnified rectangular section is given below the images to enable a clearer view of the automatically detected (in red) myofibrillar disruptions in the Actn3Ko compared with the absence of such deviations in the WT fibre. b Group analysis in a substantial number of single fibres from several animals reveals significantly higher VD values in Actn3KO fibres over fibres from WT littermates, indicative of a higher linear out-of-register disorder. As for the angular variability of myofibrils, the CAS values were similar in both groups. **p < 0.001, Mann–Whitney rank sum test. (n/m): n single fibres from m animals

Back to article page

Skeletal Muscle

ISSN: 2044-5040

Contact us