Fig. 8From: The influence of age, sex, and exercise on autophagy, mitophagy, and lysosome biogenesis in skeletal muscleRegulation of lysosome biosynthetic pathways in young and aged, male and female mouse muscle with exercise. A Representative western blots for TFEB and TFE3 protein. B Quantification of TFEB protein in combined and sex-separated groups. C Quantification of TFE3 protein in combined and sex-separated groups. D Representative western blots for TFEB protein in nuclear and cytosolic fractions in sedentary and exercised, young and aged, male and female mice. E % nuclear TFEB protein in combined and sex-separated male and female mice. F Fold-change in nuclear TFEB protein in each group examined. G TFEB promoter activity (luciferase; RLU) in young and aged, sedentary, and exercised mice. Values in B and C were corrected to GAPDH and are reported as means ± SEM. N = 10/male group, 8/female group. Line break in representative blot is different sections from the same blot. In D–F, cytosolic values were corrected to α-tubulin and nuclear values were corrected to H2B and reported as mean ± SEM, in A.U. N = 5/male group, 4/female group. In G, N = 6/group. The main effects of 2-way ANOVA are represented on graph at p < 0.05. δ p < 0.05, post hoc significance. *p < 0.05, t-test between indicated groupsBack to article page