Fig. 2
From: Dysregulation of Tweak and Fn14 in skeletal muscle of spinal muscular atrophy mice
Aberrant expression of Tweak and Fn14 in skeletal muscle of Smn2B/−SMA mice. a–g qPCR analysis of parvalbumin (a), Tweak (b), Fn14 (c), Pgc-1α (d), Mef2d (e), Glut-4 (f), and HKII (g) in TA muscles from P0 (birth), P2 (pre-symptomatic), P4 (pre-symptomatic), P11 (early symptomatic), and P19 (end stage) Smn2B/− and WT mice. Normalized relative expressions are compared to WT P0. Data are mean ± SEM, n = 3–4 animals per experimental group, two-way ANOVA, Sidak’s multiple comparison test between genotypes, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. h Quantification of Tweak protein levels normalized to total protein in the triceps of late-symptomatic (P18) Smn2B/− mice and age-matched WT animals. Images are representative immunoblots. Data are mean ± SEM, n = 6–7 animals per experimental group, unpaired t-test, p = 0.014. i Quantification of NF-κB1 p50 and p105 protein levels normalized to total protein in the triceps of late-symptomatic (P18) Smn2B/− mice and age-matched WT animals. Images are representative immunoblots. Data are mean ± SEM, n = 6–7 animals per experimental group, unpaired t-test, ns, not significant (p50), p = 0.0354 (p105). j Quantification of NF-κB2 p52 and p100 protein levels normalized to total protein in the triceps of late-symptomatic (P18) Smn2B/− mice and age-matched WT animals. Images are representative immunoblots. Data are mean ± SEM, n = 3–4 animals per experimental group, unpaired t test, ns, not significant (p52), p = 0.0532 (p100)