Fig. 3

Tweak and Fn14 are not dysregulated in denervated (nerve crush) muscles of pre-weaned mice. A sciatic nerve crush was performed on postnatal day (P) 7 WT FVB/N mice, and both ipsilateral (nerve crush) and contralateral (control) TA muscles were harvested at P14. a Representative images of hematoxylin and eosin-stained cross sections of control and nerve crush TA muscles. Scale bars, 100 μm. b Myofiber area in control and nerve crush TA muscles. Data are mean ± SEM, n = 3–6 animals per experimental group, unpaired t-test, p = 0.0020. c Myofiber size distribution in control and nerve crush TA muscles. d qPCR analysis of parvalbumin, Tweak, Fn14, Pgc-1α, Mef2d, Glut-4, HKII, Klf15, and Smn in control and nerve crush TA muscles. Normalized relative expressions for each gene are compared to control muscle. Data are mean ± SEM, n = 4–6 animals per experimental group, two-way ANOVA, uncorrected Fisher’s LSD, ns, not significant