Fig. 4

Tweak and Fn14 are dysregulated in cardiotoxin-induced muscle necrosis in pre-weaned mice. Cardiotoxin was injected in the left TA muscle of postnatal day (P) 10. The right TA muscle was injected with equal volumes of 0.9% saline. TA muscles were harvested 6 days later. a Representative images of hematoxylin and eosin-stained cross sections of saline- and cardiotoxin-injected TA muscles. Scale bars, 100 μm. b Percentage of muscle fibers with centrally located nuclei in saline- and cardiotoxin-injected TA muscles. Data are mean ± SEM, n = 3 animals per experimental group, unpaired t--test, p = 0.0020. c qPCR analysis of parvalbumin, Tweak, Fn14, Pgc-1α, Mef2d, Glut-4, HKII, Klf15, and Smn in saline- and cardiotoxin-injected TA muscles. Normalized relative expressions for each gene are compared to saline-treated muscle. Data are mean ± SEM, n = 3 animals per experimental group, two-way ANOVA, uncorrected Fisher’s LSD, ns, not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001