Dysregulation of Tweak and Fn14 in skeletal muscle of spinal muscular atrophy mice

Meijboom, Katharina E.; Sutton, Emma R.; McCallion, Eve; McFall, Emily; Anthony, Daniel; Edwards, Benjamin; Kubinski, Sabrina; Tapken, Ines; Bünermann, Ines; Hazell, Gareth; Ahlskog, Nina; Claus, Peter; Davies, Kay E.; Kothary, Rashmi; Wood, Matthew J. A.; Bowerman, Melissa

doi:10.1186/s13395-022-00301-z

Skeletal Muscle

Table 2 Glucose metabolism genes significantly dysregulated in the same direction in triceps and quadriceps of P7 Smn^−/−;SMN2,⁻Fn14^−/− and Tweak^−/− mice when compared to P7 WT mice

From: Dysregulation of Tweak and Fn14 in skeletal muscle of spinal muscular atrophy mice

		*Triceps*						*Quadriceps*
Gene		*Smn^−/−;SMN2*		*Fn14*^−/−		*Tweak*^−/−		*Smn^−/−;SMN2*		*Fn14*^−/−		*Tweak*^−/−
Gene		*Fold change*	*p-value*	*Fold change*	*p-value*	*Fold change*	*p-value*	*Fold change*	*p-value*	*Fold change*	*p-value*	*Fold change*	*p-value*
1,4-Alpha-glucan branching enzyme 1 (Gbe1)	Down	1.2158	0.001826	−1.1649	0.00362		ns	↑		−1.3664	0.00073	−1.648	0.002807
Dihydrolipoamide S-succinyltransferase (Dlst)	Down	−1.4834	0.000409	−1.1593	0.000992	−1.5512	0.009639	−1.7555	0.000001	−1.2472	0.000422	−1.4384	0.000389
Enolase 1 (Eno1)	Down	−2.8937	0	−1.2057	0.001592	−1.2553	0.012037	−2.8182	0	−1.3798	0.000748	−1.6093	0.000595
Filamin B (Fh1)	Down	−1.2988	0.002491	−1.11	0.011675	−1.2603	0.044285	−1.4732	0.000033	−1.1362	0.010963		ns
Fructose-bisphosphatase 2 (Fbp2)	Up	1.3862	0.002522	1.6462	0.003035	1.4036	0.004574	1.5193	0.000348	1.4564	0.00028		ns
Glycogen hosphorylase muscle-associated (Pygm)	Down	−1.2185	0.002346		ns		ns	−1.2577	0.000493	−1.1492	0.034146	−1.1388	0.045356
Isocitrate dehydrogenase 3 (NAD(+)) alpha (Idh3a)	Down	−1.3412	0.014566	−1.0994	0.022547	−1.2728	0.007865	−1.6021	0.000063	−1.3412	0.002972	−1.288	0.023111
Isocitrate dehydrogenase 3 (NAD(+)) Beta (Idh3b)	Down	−1.2006	0.00032	−1.1227	0.017864		ns	−1.3376	0.00003	−1.1815	0.001146	−1.1462	0.018887
Oxoglutarate dehydrogenase (Ogdh)	Down	−1.184	0.032659	−1.0914	0.009317	−1.3354	0.001844	−1.295	0.000068	−1.2062	0.010753	−1.4183	0.011564
Phosphofructokinase, liver type (Pfkl)	Down	−1.3403	0.002443		ns		ns	−1.8003	0.033611	−1.2159	0.003577	−1.699	0.00477
Pyruvate dehydrogenase E1 alpha 1 subunit (Pdha1)	Down		ns	−1.1094	0.005985	−1.1421	0.040785	−1.1915	0.029091	−1.2056	0.006742	−1.2258	0.009299
Pyruvate dehydrogenase kinase 4 (Pdk4)	Up	7.4434	0.002676	1.6982	0.017988	1.7597	0.03066	5.3286	0.038343	1.7406	0.003824	1.556	0.006172
Phosphoglycerate kinase 1 (Pgk1)	Down	−1.7093	0.000041	−1.1129	0.006326	−1.1699	0.049145	−1.7605	0	−1.3544	0.006063		ns
Triosephosphate isomerase 1 (Tpi1)	Down	−1.8595	0.000342		ns		ns	−1.8225	0.000006	−1.2313	0.002462	−1.2697	0.011066

Ns not significant, ↑ significantly upregulated. Fold change (2^(-delta-delta CT)) is the normalized gene expression (2^(-delta CT)) in the test sample divided the normalized gene expression (2^(-delta CT)) in the WT samples. Fold-change values greater than 1 indicate a positive- or an upregulation and the fold regulation is equal to the fold change. Fold-change values less than 1 indicate an negative or downregulation and the fold regulation is the negative inverse of the fold change. The p-values are calculated based on a Student’s t-test of the replicate 2^(-delta CT) values for each gene in the control group and treatment groups

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