Fig. 2
Increased ROS/iNOS-derived NO after denervation upregulated p53/progerin. A Flow cytometric analysis of ROS in the control WT peripheral blood group 3, 7, and 14 days after denervation. B Dynamic changes in ROS in the peripheral blood. *P<0.05 versus hour 0 (n=6/group). C Flow cytometric analysis of ROS in the control WT gastrocnemius muscles 3, 7, and 14 days after denervation. D Dynamic changes in ROS in denervated gastrocnemius muscles. *P<0.05 and **P<0.01 versus hour 0 (n=6/group). E Elevated expression of iNOS mRNA in the WT group of mice after denervation was determined by qPCR. Level of elevation of INOS of mice in the Den group was much higher than that of those in the control group. *P<0.05 versus control (n=6/group). F INOS protein levels determined by western blotting showed a trend similar to that of mRNA expression. *P<0.05 versus control (n=6/group). G–H The expression of progerin in the control, Den-saline, and the Den-anti-ROS/iNOS group of mice was determined by western blotting. Level of elevation of progerin in the Den-saline group of mice was much higher than that in the Den-anti-ROS/iNOS group. *P<0.05 versus control; #P<0.05 versus Den-saline (n=6/group). I–J The expression of P53 in the WT group of mice after denervation was determined by western blotting. Level of elevation of P53 of the Den-saline group of mice was much higher than that of the Den-anti-ROS group. Levels of elevation of P53 and ac-p53 of the Den-saline group were much higher than those of the Den-anti-iNOS group. *P<0.05 versus control; #P<0.05 versus Den-anti-saline (n=6/group)