Fig. 1

Food intake and morphometrical analyses of masseter and tongue muscles of WT and DMD rats. A The amount of food intake per day was measured at 3 and 7 months old. The data are expressed as mean ± SE. **, p < 0.01 by unpaired Student’s t-test. B The masseter muscle was considered as an ellipse (dotted white circle) and its long and short diameters were measured with calipers to calculate the area. C Changes in the area of the masseter muscle at 1, 3, 6, and 8 months old. The data are expressed as mean ± SE. **, p < 0.01 by unpaired Student’s t-test. D The tongue was cut at the root (dotted black line) (left) and the width (dotted black line) and area (dotted red circle) were measured. Scale bar = 1 cm. E Changes in the width and cross-sectional area of the tongue at 1, 3, and 8 months old. The data are expressed as mean ± SE. **, p < 0.01 by unpaired Student’s t-test