Fig. 4

Quantitative analyses of gene expression and satellite cells in muscles of WT and DMD rats. A p16, B p19, C p21, and D p53 transcriptions in the masseter and tongue muscle at 9 months old. The data are expressed as mean ± SE. * and **, p < 0.05 and p < 0.01 by unpaired Student’s t-test, respectively (WT, n = 5; DMD, n = 6). E Number of Pax7-positive satellite cells in the sections at 9 months old. The data are expressed as mean ± SE. There was no significant difference among all groups by Tukey–Kramer test (WT, n = 5; DMD, n = 6). F, G Relative number of Pax7- and MyoD-positive satellite cells from 9-month-old rats after 4-day culture in vitro. The data are expressed as mean ± SE. * and **, p < 0.05 and p < 0.01 by unpaired Student’s t-test, respectively (WT, n = 3; DMD, n = 3). H Utrn transcription in the masseter and tongue muscle at 9 months old. The data are expressed as mean ± SE. *, p < 0.05 by unpaired Student’s t-test (WT, n = 5; DMD, n = 6)