Skip to main content
Fig. 1 | Skeletal Muscle

Fig. 1

From: The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Fig. 1

DUX4C and DUX4L26 gene maps and DUX4C alternative intronic transcripts. Comparison of the gene locations on chromosome 3p12.3 and 4q35 regions presenting either DUXL26 next to FRG2C (A) or DUX4C next to FRG2 (B). (B, bottom) Schematic representation of the endogenous DUX4C 3’ UTR sequences found using RT-PCR in primary and immortalized human myoblasts (some amplified fragments are shown in Fig. S1). The intron 2 was previously identified in C2C12 cells transfected with a DUX4C genomic construct [3]. C The table summarizes the donor (DS) and acceptor (AS) splice sites sequences and their coordinates (respectively, last 3′ and first 5′ nucleotide position in exon) on Genbank #AF146191. All correspond to canonical (or reported atypical*) splice sites. The letters in bold correspond to the highest nucleotide frequency in the corresponding consensus position. In red, the 5′ and 3′ intron sequences used in splice site classifications [11]. The complete DUX4C sequences obtained from several cell cultures after its cDNA cloning are available in Table S2. PAS: polyadenylation signal

Back to article page