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Fig. 3 | Skeletal Muscle

Fig. 3

From: Angiogenesis precedes myogenesis during regeneration following biopsy injury of skeletal muscle

Fig. 3

Tile scanned images showing the time course of microvascular and myofiber regeneration after punch biopsy. A At 7 dpi, angiogenesis begins around edges of wound. B At 14 dpi, regenerating microvessels have spanned the wound and myofibers have begun regenerating into the wound from severed ends. C At 21 dpi, myofibers traverse the wound while capillary networks remain disorganized. Dark areas within the regenerated area reflect interweaving among adjacent myofibers, which contrasts with the parallel (flat) organization of surrounding (uninjured) myofibers. Within the wound, microvessels remodel (arrowhead) to supply a cluster of adipocytes (not visible) where myofibers have not regenerated. D, E, and F are higher magnification of regions within broken yellow squares shown in A, B, and C to illustrate angiogenesis at the wound edge, microvascular branches extending across the gap, and microvascular remodeling around a cluster of adipocytes, respectively. Images are from Cdh5-mTmG mice in which ECs are green (GFP) and all other cells are red (tdTomato)

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