Fig. 2

Myomaker expression in myofibers is associated with myofiber membrane damage and reduced myofiber stiffness. A Serum CK levels are elevated after 3 days of Myomaker induction in myofibers. B Representative images of immunofluorescence staining for IgM in the rectus femoris after 3 days of Myomaker induction reveal an elevated proportion of IgM⁺ myofibers compared to the control. Quantification of the percentage of IgM⁺ myofibers in the soleus, EDL, TA, and rectus femoris is shown below the images. Scale bar = 100 μm. C Atomic force microscopy on isolated myofibers reveals reduced myofiber stiffness with Myomaker expression in myofibers 3 days after induction. Myofiber stiffness in dystrophic myofibers is restored to wild-type levels after ablation of Myomaker from myofibers. Each bar represents the average stiffness of three myofibers from a given mouse. Statistical analyses and presentation: data are presented as mean ± SEM. A and B Two-tailed Student’s t-test; *P < 0.05, ****P < 0.0001. C One-way ANOVA between experimental groups with a Tukey’s post hoc test; **P < 0.01, ***P < 0.001