Fig. 3

Long-term Myomaker expression in myofibers leads to elevated centrally nucleated myofibers and muscle atrophy. A Serum CK levels are not elevated after 12 weeks of Myomaker expression in myofibers. B Immunofluorescence staining for IgM in the rectus femoris after 12 weeks of Myomaker induction. Quantification of the percentage of IgM⁺ myofibers in the soleus, TA, and rectus femoris is shown below the images. Scale bar = 100 μm. C Quantification of myofibers with centrally localized nuclei in the soleus, TA, and rectus femoris reveals elevated levels of regeneration with Myomaker expression in myofibers. D Muscle mass to tibia length ratios of the rectus femoris and TA were reduced after 12 weeks of Myomaker expression in myofibers. E Myofiber size, quantified by minimum Feret’s diameter, was quantified in the soleus, TA, and rectus femoris. Statistical analyses and presentation: data are presented as mean ± SEM. C-E two-tailed Student’s t-test; *P < 0.05, **P < 0.01