Ca2+ permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca2+ signaling to sustain muscle function
- Chang Seok Lee1,
- Adan Dagnino-Acosta1,
- Viktor Yarotskyy2,
- Amy Hanna1,
- Alla Lyfenko2,
- Mark Knoblauch1,
- Dimitra K Georgiou1,
- Ross A Poché1,
- Michael W Swank1,
- Cheng Long1,
- Iskander I Ismailov1,
- Johanna Lanner1,
- Ted Tran1,
- KeKe Dong1,
- George G Rodney1,
- Mary E Dickinson1,
- Christine Beeton1,
- Pumin Zhang1,
- Robert T Dirksen2 and
- Susan L Hamilton1Email author
© Lee et al.; licensee BioMed Central. 2015
Received: 23 October 2014
Accepted: 11 December 2014
Published: 29 January 2015
Ca2+ influx through CaV1.1 is not required for skeletal muscle excitation-contraction coupling, but whether Ca2+ permeation through CaV1.1 during sustained muscle activity plays a functional role in mammalian skeletal muscle has not been assessed.
We generated a mouse with a Ca2+ binding and/or permeation defect in the voltage-dependent Ca2+ channel, CaV1.1, and used Ca2+ imaging, western blotting, immunohistochemistry, proximity ligation assays, SUnSET analysis of protein synthesis, and Ca2+ imaging techniques to define pathways modulated by Ca2+ binding and/or permeation of CaV1.1. We also assessed fiber type distributions, cross-sectional area, and force frequency and fatigue in isolated muscles.
Using mice with a pore mutation in CaV1.1 required for Ca2+ binding and/or permeation (E1014K, EK), we demonstrate that CaV1.1 opening is coupled to CaMKII activation and refilling of sarcoplasmic reticulum Ca2+ stores during sustained activity. Decreases in these Ca2+-dependent enzyme activities alter downstream signaling pathways (Ras/Erk/mTORC1) that lead to decreased muscle protein synthesis. The physiological consequences of the permeation and/or Ca2+ binding defect in CaV1.1 are increased fatigue, decreased fiber size, and increased Type IIb fibers.
While not essential for excitation-contraction coupling, Ca2+ binding and/or permeation via the CaV1.1 pore plays an important modulatory role in muscle performance.
KeywordsCaV1.1 CaM kinase II Fatigue Fiber type Protein synthesis and Skeletal muscle
Excitation-contraction coupling (ECC) in skeletal muscle involves a mechanical interaction between the L-type voltage-dependent Ca2+ channel (CaV1.1) and the sarcoplasmic reticulum (SR) Ca2+ release channel (the ryanodine receptor, RyR1). Although Ca2+ entry through CaV1.1 is not required for skeletal muscle ECC , CaV1.1 opens after the initial voltage-gated release event to allow Ca2+ to both bind and permeate the channel pore . Like other voltage-dependent Ca2+ channels, CaV1.1 undergoes multiple conformational changes driven by membrane depolarization and/or Ca2+ movement (and/or binding) through the channel [3,4]. The opening of CaV1.1 actually occurs after the opening of RyR1 , and CaV1.1 has been suggested to facilitate RyR1 closing . Although there is only limited understanding of the role of Ca2+ permeation via CaV1.1, this influx has been suggested to regulate clustering of nicotinic acetylcholine receptors at the neuromuscular junction [7-9] and muscle plasticity .
Alterations in Ca2+ binding and permeation through CaV1.1 contribute to the pathological development of several muscle diseases [11-14]. Mutations in both RyR1 and CaV1.1 are associated with malignant hyperthermia (MH) in humans. The CaV1.1 mutations either enhance Ca2+ influx, increase the Ca2+ sensitivity of RyR1, and/or destabilize a closed state of RyR1. At least one of these MH mutations (R174W) ablates the L-type current and increases the sensitivity of RyR1 to caffeine, but does not alter ECC, demonstrating that the disease mechanism involves a distinct role for CaV1.1 . Together, these findings suggest a more extensive role for Ca2+ permeation via CaV1.1 in muscle function than what is presently understood.
In skeletal muscle, as in most cells, Ca2+ regulates multiple signaling pathways depending on the amplitude, frequency, duration, and location of the Ca2+ signal (reviewed by Tavi and Westerblad ). CaMKII is a major integrator of local Ca2+ signals that exhibits a privileged interaction with L-type channels (CaV1.x) due to its ability to cluster nearby and, upon Ca2+ binding, to interact with CaV1.x channels . CaV1.x channels prevail over other Ca2+ influx channels with respect to transducing excitation-transcription coupling (ETC) [18,19]. Hudmon et al.  demonstrated that CaMKII tethers to CaV1.2 and phosphorylates the carboxy-terminal tail of the α1 subunit to increase Ca2+ influx. The interaction of CaMKII with CaV1.2, in turn, facilitates CaMKII auto-phosphorylation.
To directly delineate the role of Ca2+ permeation through CaV1.1 in skeletal muscle function, we created a mouse with a Ca2+ binding and permeation defect in CaV1.1. We demonstrate that this CaV1.1-mediated pathway utilizes the flexibility of Ca2+ signaling (location, frequency, duration, and amplitude) to regulate CaMKII and calcineurin activation, thereby enhancing SR Ca2+ store refilling and protein synthesis to modulate fatigue susceptibility, muscle size, and fiber type distribution.
N-benzyl-p-toluene sulfonamide (BTS) was purchased from Tocris Biosciences (Bristol/UK, England), latrunculin A from AdipoGen (San Diego/California, United States), and 4-chloro-m-cresol (4CmC) from Pfaltz & Bauer (West Chester/Pennsylvania, United States). The calcium dyes fura-2 AM and mag-fluo-4 AM were purchased from Invitrogen (Grand Island/New York, United States). Molecular weight marker for western blotting was purchased from GenDEPOT (Barker/TX, United States). Every other reagent including Insulin, KN-92, KN-93, and the myristoylated autocamtide 2-related inhibitory peptide (AIP) were purchased from Sigma-Aldrich (St. Louis, United States).
In this study, male mice between six and 12 weeks old were used (The mice were generated by the Hamilton lab and backcrossed with the C57BL/6 J mice obtained from Jackson’s Laboratory (Bar Harbor/Maine, United States)), unless otherwise indicated. All mice were housed at room temperature with a 12:12 hour light-dark cycle and provided with food and water ad libitum. All procedures were approved by the Animal Care Committees at Baylor College of Medicine (Texas, United States) and the University of Rochester (New York, United States).
Creation of E1014K mice
The EK mutation was engineered on a genomic fragment of about 500 bp containing the exon-encoding residue E1014. A tetracycline resistance gene cassette (tet, for bacteria selection) and a neomycin cassette (neo, for ES cell selection) were inserted into the middle of the engineered fragment, flanked by about 250 bp homologies to CaV1.1. This selection marker-containing fragment was used to isolate a larger CaV1.1 genomic clone from a mouse 129 phage library via homologous recombination in Escherichia coli . Several clones were isolated and we chose one with an appropriate length of homologies on each side of the two cassettes for electroporation into AB2.2 ES from the 129SvEv cells. Recombinant ES clones were identified using southern blot analysis, and one of the clones was injected into blastocysts derived from C57BL/6 J mice to produce chimeras. The targeted allele was germ-line transmitted and the two selection cassettes were removed through crosses with Meox2-Cre mice .
To expedite the transfer of the E1014K mutation from the 129SvEv mouse sub-strain background to a congenic C57BL/6 J background, speed congenics were used, in addition to conventional backcrossing. We identified 92 microsatellites of maximal base-pair length disparity between the 129SvEv and C57BL/6 J strains. These microsatellites were chosen to representatively span the entire genome and show distinct electrophoretic separation. Primer pairs were selected to PCR amplify the chosen microsatellites to be resolved by electrophoresis in Spreadex EL300 Wide Mini Gels (Elchrom Scientific, Cham, Switzerland). Finally, we compared 129SvEv and C57BL/6 J DNA standards to DNA from our backcrossed mice, and selected mice with the most sequence homology to C57BL/6 J DNA to be used in the next backcross. This method both ensured congenicity between the wild-type (WT) and EK mutant mice and provided a means to more quickly begin experimentation.
Isolation of flexor digitorum brevis muscle fibers
Skeletal muscle fibers were isolated from the flexor digitorum brevis (FDB) muscle obtained from WT and EK mice as described .
Whole-cell patch clamp recordings of CaV1.1 currents
CaV1.1 currents were analyzed using Igor Pro 6 (Lake Oswego, Oregon, United States) and Clampfit 9 (Sunnyvale, California, United States) software.
Measurements of electrically-evoked Ca2+ release in flexor digitorum brevis muscle fibers stimulated during a single twitch
Acutely isolated FDB fibers were loaded for 20 minutes at room temperature with 4 μM mag-fluo-4 AM in a Kreb’s Ringer solution containing (in mM): 146 NaCl, 5 KCl, 2 CaCl2, 1 MgCl2, and 10 HEPES, at pH 7.4. Fibers were then washed and incubated for 20 minutes in dye-free Ringer’s solution supplemented with 20 μM BTS, a skeletal muscle myosin inhibitor, to block contraction. Mag-fluo-4 AM-loaded FDB fibers were excited at 480 ± 15 nm and fluorescence emission detected at 535 ± 20 nm was collected at 10 kHz using a photomultiplier system. Electrical field stimulation (8 V, 1 ms, and 10 stimuli at 1 Hz) was elicited using a glass electrode placed adjacent to the cell of interest. Peak changes in mag-fluo-4 fluorescence for all 10 stimuli were measured as (Fmax-F0)/F0 and then averaged to generate a single peak value for each fiber. The rate of mag-fluo-4 fluorescence decay for the second, third, and fourth twitches for each fiber was fitted to a first order exponential function and the resulting amplitude and tau values were averaged.
Mn2+ quench measurements
Mn2+ quench of fura-2 emission was measured in myotubes loaded with 5 μM fura-2 AM for 1 h at 37°C in Kreb’s Ringer solution. Briefly, prior to Mn2+ quench measurements, myotubes (primary cultured cells from muscle of mice) were treated with 100 μM ryanodine to block RyR1-mediated Ca2+ release during subsequent KCl application. Fura-2-loaded myotubes were excited at the experimentally determined fura-2 isosbestic point (362 nm) and emission monitored at 510 nm during perfusion of 50 mM KCl in the presence of 0.5 mM Mn2+. Maximum rates of fura-2 quench during KCl application were determined and evaluated for statistical significance.
Calcium imaging in confocal line scan mode in flexor digitorum brevis muscle fibers stimulated with a single 50 Hz train
To monitor Ca2+ release during electrical stimulation, FDBs fiber were loaded with 5 μM of mag-fluo-4 AM for 30 minutes at room temperature in the presence of 20 μM of BTS. Loaded FDBs were placed on the stage of a confocal microscope with an adapted perfusion system (tyrode with 20 μM BTS at 0.5 mL/min) and imaged in line scan mode using the 20x objective (EC Plan-Neofluar) mounted in the confocal microscope (Zeiss LSM 510 meta, California, United States), one line was acquired every 1.15 milliseconds (3.66 μsec/pixel time) using the 488 nm excitation laser and the LP 505 emission filter (Zeiss, California, United States). FDBs were stimulated with 50 square electrical pulses (200 μsec duration) at 50 Hz and the produced florescence transients was normalized (F/F0) and plotted.
Measurement of Ca2+ transients during repetitive stimulation with 100 Hz trains
Isolated FDB fibers were loaded with 5 μM of mag-fluo-4-AM for 30 minutes at room temperature, followed by washout with fresh DMEM (Life technologies, NY, United States). Electrical stimulation was performed using two platinum wires placed at each side of the fiber oriented longitudinally and fatigue was induced with uninterrupted application of electrical trains (100 Hz, 250 ms, every 1.5 seconds; 0.17 duty cycle) for 300 seconds. For evaluation of RyR1-releasable SR Ca2+ store content, 1 mM of 4CmC was perfused at 3.25 ml/min, applied after 60 trains of electrical stimulation. Mag-fluo-4 fluorescence was collected at 20 Hz. Data were collected and analyzed using Metafluor version 6.2 software (Molecular Devices, California, United States).
Muscles were homogenized and lysed in ice-cold RIPA buffer consisting of (mM): 25 Tris pH 7.6, 150 NaCl, 1 Na3VO4, 10 NaPyroPO4, 10 β-glycerophosphate, 10 NaF, PMSF, protease inhibitor cocktail (Santa Cruz), 1% NP40, 1% sodium deoxycholate, and 0.1% SDS (Every reagents came from Sigma Aldrich, St. Louis, United States). Equal amounts of total protein from whole muscle lysates were resolved by electrophoresis, transferred to PVDF (Millipore, Billercia, United States) membrane and western blot analyses were performed using antibodies shown in Additional file 1: Table S1. LI-COR IRDye™ infrared dyes were used as secondary antibodies and immunoreactive bands were visualized using the Odyssey Infrared Imaging System (LI-COR) (LI-COR Inc, Lincoln, United States). To allow the use of data from multiple western blots, the fluorescent band intensity of each band within a single western was first normalized to GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) as a loading control and then calculated as %WT average from that specific western blot. Data were then pooled to give %WT ± SEM.
Co-localization studies and single fiber immunocytochemistry
Single FDB fibers plated on glass slides were fixed with 2% paraformaldehyde in 0.1 M phosphate buffer (PB) (21.6 mM Na2HPO4 and 81.4 mM NaH2PO4, pH7.2) for one hour at room temperature, washed twice with phosphate buffered saline (PBS) (3.8 mM NaH2PO4, 16.2 mM Na2HPO4, 150 mM NaCl, pH 7.4), permeabilized, and blocked in PBS containing 0.5% Triton X-100 and 5% BSA overnight at 4°C (All reagents come from Sigma Aldrich as described in Materials). Primary antibodies diluted in PBST (PBS containing 0.5% TX-100) were added to slides and incubated overnight at 4°C. After washing twice with PBS, Alexa-fluor conjugated antibodies were added. Fibers were washed three times with PBS for 10 minutes each and mounted in Fluoromount-G (SouthernBiotech, Birmingham, United States). Fibers were imaged using a Zeiss LSM 510 META confocal microscope with a 100x/1.30NA oil lens, HeNe 543 nm laser, and Argon 458,477,488,514 laser (Zeiss, California, United States).
Proximity ligation assay
We used proximity ligation assays (PLAs) to identify proteins that are within 40 Å of each other. PLA was performed on single FDB fibers plated on glass disks. Fibers were kept at 37°C in a 95% O2-5% CO2 incubator in DMEM solution supplemented with 10% FBS. Fibers were then fixed with 2% paraformaldehyde in 0.1 M PB and incubated with the primary antibodies. The PLA was performed with the Duolink kit (Olink Biosciences, Uppsala, Sweden) according to the instruction of the user manual using an anti-goat MINUS PLA and anti-rabbit PLUS PLA probes and the orange detection reagent (Cy3) (Olink Biosciences, Uppsala, Sweden). Fibers were imaged with confocal microscopy (Zeiss LSM 510 META, with a 100x/1.30NA oil lens and HeNe 543 nm laser). For analysis, Z stacks were projected and saved as a single image. The positive spots were counted with Image J (8-bit images filtered with a Gaussian Blur filter (Rasband, W.S Image J, U.S. National Institutes of Health, Bethesda, Maryland, United States), σ = 1, and same threshold per set adjusted at 15-30). The number of spots counted was normalized to the area of the fiber estimated from the width and length of the fiber in the image. For each set of experiments, the average counts of WT fibers (control) were set to 100% and the number of spots in each experimental condition was calculated as percentage change.
Eight-week-old WT and EK mice were fasted for 12 hours and then given an intraperitoneal injection of insulin (1 U/kg) diluted in saline. Control mice were injected with saline. After 7.5 minutes mice were sacrificed and muscles (soleus and EDL (Extensor Digitorum Longus)) were isolated, frozen in liquid nitrogen, and stored at −80°C until use. Muscle levels of pAkt1/2 and pGSK3β in the presence and absence of insulin were measured as described in Butler et al. .
Detection of puromycin-labelled proteins
For measurement of protein synthesis we used an in vivo SUnSET technique [24,25]. Briefly, mice (13 weeks of age) were food deprived for eight hours. Propofol (18 μl/g) (Abbott Laboratories, North Chicago, United States) was administered via an intraperitoneal injection 15 minutes before the puromycin injection. The mice were then given an intraperitoneal injection of puromycin (0.04 μmol/g BW) and sacrificed 35 minutes later. At 10 minutes before sacrificing, insulin or saline (control) was administered via intraperitoneal injection. Muscles were isolated, homogenized, and prepared for western blotting with anti-puromycin antibody. For normalization to total protein, the same western blots were stained with Swift Membrane Stain™ kit (G-Biosciences, St. Louis, United States).
Ras activity was measured using a Ras activity assay kit (Cytoskeleton, Denver, United States). Briefly, muscle was lysed in buffer and protein concentration was measured. Raf-Ras binding domain (RBD) beads (50 μl) were added to the muscle lysates (total 500 μl of 2 mg/ml lysates) and the mixture was incubated at 4°C on a rotator for one hour. After incubation, Raf-RBD beads were pelleted by centrifugation at 5,000 × g at 4°C for one minute and washed with wash buffer. The bound active Ras was eluted in the two × sample buffer by boiling for three minutes. Eluted protein was run on 12% gel, transferred to PVDF membrane, and immunoblotted with Ras-specific antibody. The westerns were normalized to the amount of GAPDH in the muscle lysates (60 μg).
Sarco/endoplasmic reticulum Ca2+-ATPase activity
Electrical stimulation of isolated muscle for signaling changes
To assess stimulation-induced changes in signaling pathways we used the method of Sakamoto et al. . Intact soleus and EDL muscles were removed and suspended between a force transducer and stationary anchor within a test chamber filled with warmed (35°C) Kreb’s Ringer solution (KRS) oxygenated with a 95/5% mixture of O2/CO2, as above. After a 30 minute resting equilibration period, muscles to be stimulated underwent a fatigue protocol (100 Hz, 200 ms train duration, one second intervals) for five minutes per muscle. At the completion of the stimulation protocol, rested and stimulated muscles were immediately frozen in liquid N2 and stored at −80°C. For muscles treated with KN-93, the drug was added to the chamber at the start of the 30 minutes equilibration period, at a final concentration of 5 μM.
Muscle force frequency and fatigue
Intact soleus and EDL muscles were removed and immediately immersed in incubation medium comprised of KRS (oxygenated with a 95/5% mixture of O2/CO2. Muscles were tied with sutures at the musculotendinous junction and suspended between a force transducer and stationary anchor within a test chamber filled with warmed (35°C), oxygenated incubation medium. After a 20 minutes rest to allow mounted muscles to equilibrate, muscle optimal length (l o ) was determined via single-twitch force generation measurements. Next, force frequency measurements were obtained at l o using frequencies from 15 to 300 Hz at 200 ms/train followed by a fatigue protocol performed over five minutes per muscle. The specific fatigue protocol for each muscle used was for the soleus: 15 Hz, 200 ms duration, one second intervals) and for EDL: 60 Hz, 200 ms duration, one second intervals. Muscle stimulation occurred within the test chamber using platinum electrodes attached to a Grass S48 stimulator and recorded within Chart5 (version 5.2) software (eDAQ Inc, Colorado Springs, United States).
Fibertyping with cryosections and immunostaining
Skeletal muscles (soleus and EDL) were dissected, embedded in OCT compound (Tissue-Tek, Torrance, United States), and frozen in 2-methylbutane (Sigma Aldrich, St. Louis, United States) precooled in liquid nitrogen. The frozen muscles were sectioned with 10-μm thickness using a SHANDON cryostat microtome (Thermo Electron Corporation, Madison, United States). Immunofluorescent staining was performed using specific antibodies against myosin heavy chain I (MHCI), IIa (MHCIIa), and IIb (MHCIIb) (DSHB, Iowa City, USA). Briefly, sections were rehydrated with PBS for 10 minutes, followed by incubation at 4°C overnight with MHCI (BA-F8, IgG2b), MHCIIa (SC-71, IgG1), and/or MHCIIb (BF-F3, IgGM) antibodies diluted 1:50 in PBS. After washing with PBS, muscle sections were incubated at room temperature for 90 minutes with isotype-specific AlexaFluor-594-conjugated goat anti-mouse IgG2b, AlexaFluor-488-conjugated goat anti-mouse IgG1, and AlexaFluor-594-conjugated goat anti-mouse IgGM secondary antibodies diluted 1:200. After three consecutive washes with PBS, muscle slides were mounted with VECTASHIELD mounting media (Vector Laboratories, Burlingame, United States). Images were taken under a fluorescence microscope (Olympus America, Center Valley, United States). The relative numbers of the different fiber types were quantified and normalized by the total number of muscle fibers per field.
Fiber cross-sectional area
For cross-sectional area (CSA) calculations, 10-μm-thick frozen sections were obtained from the mid-belly area of the soleus and EDL muscles. Sections were immunostained for fiber type as described in the previous section, imaged at 10x magnification through an Olympus DP70 camera (Olympus America, Center Valley, Pennsylvania, United States), and saved in .tif format. Saved images were then imported into Photoshop CSE version 10.0 (Adobe Systems, San Jose, California, United States) for analysis. First, measurement scale was established by tracing a within-image scale bar (μm). Next, myofiber CSA was measured by tracing the external border of individual myofibers using the Magnetic Lasso tool. Myofibers exhibiting evidence of tears or processing artifacts were excluded from the analysis. Recorded measurements were then exported into Excel for analysis, with resulting CSA values reported in μm2.
We performed a statistical analyses of two groups using the Student’s t-test. P <0.05 was considered to be statistically significant. *P <0.05, **P <0.01, and ***P <0.001 were used to indicate statistical significance.
Creation of EK mice
To explore the role of Ca2+ influx via CaV1.1, we created mice with a knock-in mutation (E1014K or EK) in the pore region of CaV1.1. The mutation of a glutamate to lysine residue in the repeat III pore region of CaV1.x abolishes both Ca2+ binding to this site  and divalent permeation through the channel without altering ECC . The targeting construct and data confirming the mutation are shown in Additional file 2: Figure S1. Speed congenics were used to obtain mice on a clean C57BL/6 J background. The mice were homozygous viable with no immediately obvious changes in phenotype (see below).
Effects of the EK mutation on myofibrillar Ca2+ handling
We used a well-established Mn2+ quench of fura-2 fluorescence assay to determine the effect of the EK mutation on excitation-coupled Ca2+ entry (ECCE) . In WT myotubes, membrane depolarization induced by the addition of 50 mM KCl opens CaV1.1 channels, providing a pathway for Mn2+ entry to quench fura-2 fluorescence (Figure 1G, upper trace), with the maximum slope of KCl-induced Mn2+ quench directly reflecting Mn2+ entry through CaV1.1 channels (Figure 1H). Since the EK mutation in the CaV1.1 pore abolishes divalent ion permeation through CaV1.1 channels, KCl-induced Mn2+ quench (ECCE) is absent in myotubes from EK mice (Figure 1G, lower trace), consistent with ECCE reflecting Ca2+ entry via CaV1.1, as suggested by Bannister et al. . The EK fibers also showed a small but significant increase in baseline Ca2+ influx (Figure 1H).
We used a higher affinity Ca2+ indicator, fura-2 AM, to measure changes in cytosolic Ca2+ levels. Resting cytosolic Ca2+ levels did not differ between EK and WT fibers. However, following stimulation (same stimulation protocol as Figure 2C), cytosolic Ca2+ levels were higher in both EK and WT fibers (Figure 2E), but magnitude of the stimulation-induced increase in cytosolic Ca2+ was less in EK fibers. These findings suggest that Ca2+ permeation through CaV1.1 facilitates refilling of SR Ca2+ stores either directly or indirectly. The decreased Ca2+ transient amplitude during repetitive stimulation in EK fibers could be due to an decrease in Ca2+ influx via either store-operated or ECCE .
Pleiotropic effects of the EK mutation on Ca2+-sensitive pathways
We next assessed the effects of inhibition of CaMKII in WT fibers on refilling of SR Ca2+ stores during repetitive stimulation. We used a specific CaMKII inhibitory peptide, AIP (myristoylated CaMKII auto-inhibitory peptide) , and found that CaMKII inhibition increased the rate of decline in the amplitude of the Ca2+ transients (Figure 4H,) and reduced post-stimulation 4CmC releasable Ca2+ stores (Figure 4I) in FDB fibers from WT mice. KN-93 (but not the inactive K-92 analog) also reduced the amplitude of the Ca2+ transients in WT fibers but had no effect in EK fibers (Additional file 3: Figure S2C and D and Figure 4H). These data suggest that SR Ca2+ store refilling during repetitive stimulation in WT fibers is enhanced by CaV1.1-mediated activation of CaMKII, but this pathway is prevented by the EK mutation in CaV1.1 (Figure 4J).
We also examined the phosphorylation of Akt at S473 and T308 and found that phosphorylation of Akt at these sites were not significantly increased by electrical stimulation (Additional file 3: Figure S2E-H), suggesting that the signaling in response to repetitive stimulation is primarily through Ras/Raf activation of ERK1/2. Given these findings, we propose that Ca2+ permeation though or binding to CaV1.1 activates CaMKII which, in turn, phosphorylates Raf to activate Ras/Raf and ERK1/2. ERK1/2 then activates mTORC1 (Figure 6M). This stimulation-dependent signaling pathway is strongly blunted in EK muscle.
Together, our data provide compelling evidence that the EK mutation in CaV1.1 causes significant disruption to CaMKII and Ca2+-sensitive pathways in skeletal muscle, resulting in decreased SR store refilling and a decline in protein synthesis.
Effects of EK mutation on muscle function
Although acute changes in depolarization-induced contraction in low Ca2+ and in the presence of dihydropyridines were previously reported , the role of Ca2+ permeation through CaV1.1 in skeletal muscle has largely been ignored because Ca2+ influx through this channel is not required for ECC [2,43]. We demonstrate that Ca2+ signaling in skeletal muscle is significantly altered by a permeation defect in CaV1.1 (E1014K, EK). This mutation resulted in concurrent inhibition of multiple Ca2+-sensitive pathways. These signaling changes translated to significant effects on muscle contractile function, providing compelling evidence that Ca2+ binding and/or permeation via CaV1.1 is important for maintaining optimal muscle physiology.
The question that arises is whether the amount of Ca2+ entering the muscle fiber through CaV1.1 is adequate to directly drive these changes in intracellular Ca2+ handling during repetitive stimulation. If so, previous studies have either underestimated the magnitude and relative contribution of Ca2+ influx through CaV1.1 during repetitive stimulation of muscle, or CaV1.1 Ca2+ binding and permeation activates a pathway (such as CaMKII) that promotes Ca2+ entry through a second Ca2+ influx channel (such as ECCE, SOCE, or TRP). Another possibility is that Ca2+ binding to the selectivity filter triggers a conformational change in CaV1.1 that drives autophosphorylation of CaMKII bound to the channel (such as the C-terminus). CaMKII, activated by this mechanism, would then modulate multiple Ca2+ handling and downstream signaling pathways (SR Ca2+ stores, Ras, and mTORC1). The activation of these pathways would then alter numerous aspects of muscle physiology including fatigue, muscle fiber CSA, type II fiber type specification, and protein synthesis. Indeed, we demonstrate that autophosphorylation of CaMKII is decreased in the muscle of EK mice that have undergone repetitive stimulation, a condition that significantly enhances p-CaMKII levels in WT muscle.
The activation of different Ca2+-sensitive signaling pathways depends on the location, frequency, amplitude, and duration of the Ca2+ signal (reviewed by Tavi and Westerblad ). Important signaling molecules activated by Ca2+ use different combinations of these mechanisms. We demonstrate that CaMKII is localized close to CaV1.1 and that the EK mutation decreases the proximity of CaMKII to CaV1.1. Ca2+ permeation through CaV1.1, together with CaMKII activation in WT fibers, slows the decline of the Ca2+ transient with repetitive stimulation by enhancing Ca2+ store refilling. This mechanism is deficient in fibers from EK mice. CaMKII activation is decreased when Ca2+ cannot permeate CaV1.1, suggesting that Ca2+ binding within and/or movement through the CaV1.1 pore is important for activation of CaMKII. Reciprocally, CaMKII appears to amplify Ca2+ influx (either through CaV1.1 or a closely associated CaMKII-sensitive Ca2+ entry channel), since Ca2+ store refilling is decreased by CaMKII inhibitors in WT, but not EK fibers. However, at this point it is unclear whether the observed reduction in activity-dependent SR Ca2+ store content in FDB fibers from EK mice is due to loss of CaV1.1 Ca2+ influx or a downstream effect of CaMKII activation on SR Ca2+ reuptake and/or store-operated Ca2+ entry.
One of the major findings in this study was a substantial decrease in insulin-mediated protein synthesis in EK muscle, as assessed by puromycin labelling and immunoblot analysis of the mTOR signaling pathway. The mTORC1 pathway activation in response to electrical stimulation is also decreased in EK compared to WT muscle. Decreased protein synthesis in response to growth factors such as insulin and repetitive stimulation may underlie the observed decrease in muscle fiber size in EK muscle. Activation of downstream substrates of mTORC1 was significantly decreased in EK muscle, as was activation of the upstream regulators of mTOR, Akt, and ERK1/2. We suggest that the CaV1.1/CaMKII-dependent event that modulates insulin-mediated protein synthesis is upstream of PDK1 and/or mTORC2 activation, since phosphorylation of both Akt T308 (a PDK1 target) and Akt-S473 (an mTORC2 target) [44,45] are decreased in EK muscle following insulin treatment. This hypothesis is supported by previous studies that have reported Ca2+-dependent regulation of the PI3K/Akt/mTOR and Ras/Raf/ERK pathways [35-39].
The phenotypic changes associated with the EK mutation do not arise from any obvious changes in the neuromuscular junction. Chen et al.  found that a decrease in Ca2+ influx associated with a deficiency in the β-subunit of CaV1.1 altered the formation of the neuromuscular junction. This may reflect a recently identified transcriptional role for the β1a-subunit , as opposed to its more conventional role in controlling CaV1.1 Ca2+ currents, since mice with the EK mutation in CaV1.1 lack Ca2+ currents but do not exhibit alterations in neuromuscular junction preformatting (Additional file 4: Figure S3). We also did not detect any changes in immune function (Additional file 5: Figure S4).
While the magnitude of Ca2+ permeation through CaV1.1 is small, there is a significant impact of this pathway on muscle function. Changes include increased fatigue, decreased muscle fiber diameter, increased type IIb fiber specification, and decreased protein synthesis. Thus, our results indicate that small changes in CaV1.1 Ca2+ permeation, and CaMKII activation are amplified by changes in the activity of multiple critical downstream signaling pathways that impact a broad range of skeletal muscle functions. We propose that Ca2+ permeation through CaV1.1, while not required for ECC, modulates multiple downstream Ca2+-sensitive signaling pathways to improve muscle function.
autocamtide 2-related inhibitory peptide
calmodulin-dependent protein kinase II
L-type voltage-dependent calcium release channel
excitation-coupled calcium entry
extensor digitorum longus
mice with a glutamate to lysine mutation in the position 1014 of CaV1.1
flexor digitorum brevis
- Fura 2 AM:
fura-2 acetoxymethyl ester
Glyceraldehyde 3-phosphate dehydrogenase
Krebs Ringer solution
myosin heavy chain I
myosin heavy chain IIa
myosin heavy chain IIb
optimal cutting temperature
indicates phospho form if in front of a protein name
phosphate buffered saline
proximity ligation assay
Ras binding domain
ryanodine receptor 1
Sodium dodecyl sulfate
Sarco/endoplasmic reticulum calcium ATPase
store operated calcium entry
Transient receptor potential
This work is supported by grants from the Muscular Dystrophy Association and NIH (AR053349 to SLH and RTD, AR041802 to SLH, AR060831 to VY, and NIH T32 HL007676 to MK, JO, and JAL), postdoctoral fellowship from the Mexican Council of Science and Technology (186607) to ADA, and a postdoctoral fellowship from the Swedish Research Council to JTL.
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