Fig. 3

Dephosphorylated MSY3 accumulates in C2C12 nuclei. a Expression of MSY3 evaluated by IF with anti-MSY3 Ab, ZONAB, in C2C12 myoblasts in proliferating (30 % confluence) (IF left) and confluent (80 % confluence) growth (IF right) upon LY294002 (LY) treatment. Scale bar = 200 μm. b High magnification image (60×) of MSY3 expression (top) and Hoechst nuclei staining (bottom) in C2C12 myoblasts untreated (−) and upon LY treatment (LY). Scale bar = 60 μm. c N/C ratios of MSY3 signal in C2C12 myoblasts untreated (−) and upon LY treatment (+), evaluated as a mean ± DS for 60–80 cells from a total of five independent experiments. Quantification was performed as described in the “Methods” section. *P <0.05 by Student’s t test. d Densitometry calculations for MSY3 faster (dephosph) and slower (phosph) migration bands in nuclear (N) and cytosolic (C) compartments in untreated (−) and upon LY treatment (+), analyzed on Western blots performed as that shown in Fig S2B. Figure displays results representative from three independent RNAi experiments. *P <0.01; **P <0.001 by Student’s t test