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Figure 3 | Skeletal Muscle

Figure 3

From: Caspase-3, myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of myogenic differentiation

Figure 3

Caspase-3 inhibition and LIF both inhibit myogenic differentiation and caspase-3 activity. (A) Phase contrast images of myoblast cultures either with or without 10 ng/mL LIF or 100 μM Ac-DEVD-CHO (DEVD-CHO) treatment after 24 hours of differentiation showed qualitatively less myotubes present with LIF and Ac-DEVD-CHO and the combination of both compared to control. (B) Colormetric assay for caspase-3 proteolytic activity in cultures lysed 24 hours after differentiation. Caspase-3 activity is reduced with LIF, Ac-DEVD-CHO and the combination of LIF and Ac-DEVD-CHO. (C) Creatine kinase activity from the same lysates is also decreased with LIF, Ac-DEVD-CHO and the combination of LIF and Ac-DEVD-CHO indicating that inhibition of differentiation correlates with inhibition of caspase-3 proteolytic activity. Asterisk (*) indicates P < 0.05 and ns indicates P > 0.05 with one-way ANOVA and Bonferroni's post-hoc test for all treatments compared to each other (n = 4).

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Skeletal Muscle

ISSN: 2044-5040

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