LIF alters transcripts of genes relevant to myogenic differentiation and cell cycle progression and prevents decreases in proliferation due to differentiation. (A) Real-time qPCR to determine relative mRNA quantity of the genes for myogenin, myoD, p21 and c-fos comparing LIF-treated to control over 72 hours of differentiation. Asterisk (*) indicates P < 0.05 by pair wise fixed reallocation randomization tests with REST for LIF- treated compared to control at that specific time point (n = 4). (B) Dual immunofluorescently labelled myogenic cultures for incorporated BrdU and desmin from 0 to 48 hours after differentiation were counted and represented as a percentage of total cells positive for incorporated BrdU and fusion index (percentage of nuclei contained within desmin positive syncytia). Asterisk (*) indicates P < 0.05 with Student's t-test for LIF compared to control at that time point (n = 4).