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Figure 1 | Skeletal Muscle

Figure 1

From: Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction

Figure 1

Identification of Torpedo proteins listed in public access databases. To validate tandem mass spectrometry (MS/MS) data against species-specific sequences, spectra acquired via MS/MS analysis of electric organ fractions were analyzed by the SEQUEST algorithm in BioWorks 3.3.1 software, crossreferencing known and characterized Torpedo proteins listed in GenBank. Peptide acceptance criteria was set at ΔCn >0.1, a variable threshold of Xcorr versus charge state: Xcorr = 1.9 for z = 1, Xcorr = 2.2 for z = 2, and Xcorr = 2.5 for z = 3, protein Xcorr >40, and a peptide probability based score with a P value <0.01. Protein identifications were compared with a search against UniProtKB (Swiss-Prot and TrEMBL) release 14.0, all species, to maintain consistency with databases used and protein accession numbers reported. Proteins identified are categorized by the likelihood and appropriateness of detection based on protein subcellular location or on the quality of data on public access databases.

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Skeletal Muscle

ISSN: 2044-5040

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