Figure 4

Co-expression of neonatal myosin heavy chain (nMHC) and α-dystroglycan. Many nMHC-positive fibers (A-C) also expressed α-dystroglycan (A'-C'). An inverse staining pattern was visible in small nMHC-positive fibers. (A) As the level of nMHC decreased, (A') α-dystroglycan increased. In a group of regenerating fibers, (B) the level of nMHC was low in all but two fibers whereas (B') all but one were α-dystroglycan positive. Within mature fibers, (C) the regenerating nMHC-positive fibers also stained positive for (C') α-dystroglycan. Bar = 50 μm.