Figure 7
From: Regulation of myotube formation by the actin-binding factor drebrin
BTP2 does not block late stages of myoblast differentiation. (A) Experimental scheme outlining the treatment of C2C12 cells with BTP2 and subsequent analysis. (B) Western blot analysis of C2C12 cultures in growth medium (G), at the time of transfer to differentiation medium (DM) (time 0) or incubated in DM plus BTP2 from either time 0 or 60 hours and harvested at either 72 or 120 hours. (C) Cultures parallel to those in part (B) were fixed and stained for myosin heavy chain (MHC; red) and 4',6-diamidino-2-phenylindole (blue). (D) Quantification of the total fusion index in cultures from part (C). *P < 0.05 as compared with dimethyl sulfoxide (DMSO) control at the same time point. (E) Quantification of myotube formation. Values shown are means ± SD of triplicate determinations. *P < 0.05 as compared to the DMSO control at the same time point in the graph above.