A role for RNA post-transcriptional regulation in satellite cell activation

Skeletal Muscle

Table 5 Microarray and qPCR expression trends correlate for RNA binding proteins

Gene	Microarray	qPCR	Fold change	Correlation	Biological function
Celf4	↑	↑	2.51	Yes	Splicing factor
Pabpn1	↓	↓	−1.80	Yes	Positive mRNA regulator
Ppargc1a	↑	↑	1.34	Yes	Positive mRNA regulator
Mbnl1	↓	↓	−1.14	Yes	Splicing factor
Matr3	↓	↑	11.79	No	Negative mRNA regulator
Sfrs3	↓	↑	19.11	No	Splicing factor
Zfp36	↓	↓	−2.87	Yes	Negative mRNA regulator
Zfp36l1	↓	↓	−4.48	Yes	Negative mRNA regulator
Zfp36l2	↓	↓	−1.63	Yes	Negative mRNA regulator
Elavl1	↑	↑	3.01	Yes	Positive mRNA regulator
Cdk2	↑	↑	10.28	Yes	Promotes cell cycle entry
E2F3	↑	↑	12.90	Yes	Promotes cell cycle entry

Eight of ten RNA binding proteins and two cell cycle genes validate expression changes between wild type satellite cells isolated from uninjured TA muscle and from the TA 12 h post-muscle injury. Arrows show increase (↑) and decrease (↓) for both microarray and qPCR during satellite cell activation. Fold change is from qPCR data where positive values indicate increased expression and negative valued indicate decreased expression between satellite cells isolated from uninjured TA muscle and from the TA 12 h post-muscle injury. Quantitative PCR data is normalized to GAPDH or 18S.

ISSN: 2044-5040