Figure 5
From: TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of Activin A
Transforming growth factor-β-activated kinase (TAK)-1/p38/Activin A/SMAD3 pathway activation in a rat sarcopenia model. (A) Immunoblotting of phospho-SMAD3, phospho-TAK-1, phospho-p38 and GAPDH in samples from rats of different ages. The gastrocnemius muscle was sampled from four rats per age point (6, 18, 12 and 24 months). Shown are representative immunoblots and averaged densitometry data from the blots. (B) Analysis of Activin A β-chain mRNA taken from rat gastrocnemius muscle. Data are expressed as percentage of control (first column, 6-month-old rats) and shown as means ± SEM from four individual rats. Differences from control (first column, 6-month-old rats),*P < 0.05.