Figure 3

Validation of newly discovered p38α substrates. (A) Validation of three newly identified targets as in vitro p38α substrates was performed using full-length substrate incubated with a kinase assay buffer and p38α. (B) Wild-type peptides containing the phosphorylation site or peptides harbouring an S/T-to-A mutation of the phosphorylation site were incubated with p38α and a kinase assay buffer to confirm the location of the phosphorylation site. (C) The 158 phosphorylation sites identified were aligned, and a frequency logo was generated. p38α has a consensus phosphorylation motif of aXS/TP, where "a" is an aliphatic residue and "X" is any amino acid.