Figure 3
From: Sarcospan: a small protein with large potential for Duchenne muscular dystrophy
SSPN overexpression ameliorates dystrophic pathology in mdx mice. Transverse cryosections of quadriceps muscle from six-week old mdx and SSPN transgenic mdx (mdx3.0) mice were stained with hematoxylin and eosin (H&E) and visualized at low magnification to reveal the extensive areas of necrosis, denoted in white, in mdx muscle (A). Necrosis was significantly diminished in SSPN transgenic mdx muscle [69]. Sections were also viewed at higher magnification for evaluation of degeneration/regeneration, which is marked by central nucleation of myofibers (B). Overexpression of SSPN in mdx muscle dramatically reduced central nucleation [69]. Bar, 50 μm.