Figure 1
From: Bone marrow derived cells in adult skeletal muscle tissue in humans
Y chromosome-positive nuclei are incorporated into host muscle fibers. Microphotographs show confocal images of combined FISH (Y chromosome, bright green, arrow) and indirect, fluorescent immunohistochemistry for caveolin-3 (green). Nuclear DNA was counterstained by DAPI. (A) Microphotograph of a muscle fiber section (width Ă— height; 134.95 Ă— 134.95 ÎĽm) depicting Y chromosome-negative host nuclei and a single Y chromosome-positive donor nucleus (arrow). Caveolin-3 staining is used to visualize muscle fiber membranes. (B) provides a Z-stack (width Ă— height Ă— depth; 67.48 Ă— 67.48 Ă— 5.148 ÎĽm; 13 optical sections) of the muscle fiber in (A). Dashed lines mark muscle fiber membranes indicating that the DAPI/Y chromosome nucleus is localized inside the muscle fiber. Size bar = 10 ÎĽm. (C) A high magnification Z-stack (15.83 Ă— 15.83 Ă— 6.007 ÎĽm; 15 optical sections) of the double-stained nucleus depicted in (B) clearly shows the integration of the Y chromosome DNA in the nucleus.