Generation of transgenic lines. (A) Schematic representation of the hemagglutinin-tagged Ste20-like kinase (SLK) construct. Full-length murine SLK mutated at the ATP-binding site (K63R) is driven by 2500 bp of upstream promoter sequences derived from the human skeletal actin gene. A SV40 polyadenylation signal was also cloned downstream of SLK (not shown). The 1.9 kb Pvu II probe fragment spanning the promoter and cDNA regions is shown. (B) Representative Southern blot analysis of a full litter from line 3405 crossed with wild-type FVB/N showing the transgene signal on a Pvu II digest of tail DNA. The corresponding PCR analysis is shown below the autoradiogram.