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Figure 1 | Skeletal Muscle

Figure 1

From: Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle

Figure 1

p70S6K responds to stretch in C2C12 cells. C2C12 myotubes were cultured on silicone membranes and subjected to passive stretching for 30 min. (A-D) Representative immunoblots for P-ERK1/2, total T-ERK1/2, P-p70S6K (T389 site), tubulin, P-Akt, T-Akt, P-FAK, and T-FAK in non-stretched (NS) and stretched (S) C2C12 cells. (E-I) Quantification of activation levels. P-ERK1 and 2 were normalized to T-ERK1 and 2, respectively, P-p70S6K was normalized to tubulin, P-Akt was normalized to T-Akt, and P-FAK was normalized to T-FAK. n = 5-6 wells of C2C12 cells per group. Bars represent mean ± standard error. * Significantly different from non-stretched myotubes by unpaired T test. NS, non-stretched; S, stretched.

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Skeletal Muscle

ISSN: 2044-5040

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