Figure 4

Mac-1 staining and quantitative RT-PCR of CD11b show elevated macrophage infiltration throughout postnatal development. (A) Mac-1 immunostaining of tibialis anterior muscle sections shows high macrophage infiltration as early as postnatal week 1 in Lama2^DyW (DyW) mice. Wild-type (WT) mice had minimal macrophage presence at weeks 1 and 2 and virtually none by weeks 3 and 4. (B) Confirmation of these results by CD11b quantitative RT-PCR shows consistent seven- to elevenfold increases in expression compared to age-matched WT (P < 0.0001 by two-way analysis of variance for all groups (n = 5 for all groups)). Asterisks indicate statistical significance between age-matched DyW and WT animals, hash tags indicate significant changes within the DyW group and Delta indicate significant changes within the WT group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. These statistical representations also apply other symbols (Δ, #). Bar =100 μm.