Figure 1

Insl6-KO mice display impaired muscle regeneration in the model of cardiotoxin (CTX) injury. (a) Ten micromolar solution of CTX or equal volume of PBS was injected into to tibialis anterior (TA) muscle of Insl6-KO and wild-type mice at 2 μl/g body weight with a 29-gauge needle. Representative images are H&E-stained TA muscle sections at day 7 and day 14 post-injury. Muscle regeneration was quantified in histological sections by measuring the total myofiber cross-sectional area. Data are expressed as a percentage relative to the mean value of wild-type control mice. (b) TA muscle sections at day 7 post-injury were stained with either fluorescein isothiocyanate (FITC)-conjugated CD4 or CD8 antibody. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue). FITC-positive cells in 15 randomly selected fields were counted under a fluorescent microscope with 40x objective lens. The results are presented as mean ± SEM. ^*P < 0.05, †P < 0.01.