Figure 5

Hydrodynamic Insl6 gene delivery improves myositis in Insl6-KO mice. Ten micrograms of Insl6-Fc or Fc control plasmid vectors were dissolved in a large volume of saline and injected quickly via the tail vein as described in Methods. C protein-induced myositis (CIM) immunogens were injected the next day. (a) Mice were examined on Rotarod before and 14 days after CIM induction. The Rotarod test was performed at 10 revolutions/minute and the score was determined as described in Methods. (b and c) Total RNA was isolated from lower limb muscles at 14 days after CIM induction and subjected to cDNA synthesis. Relative transcript expression of CD4, CD8, TNF-α, and IFN-γ (n = 6 in each group) was measured by qRT-PCR. The data were normalized by 36B4. The results are presented as means ± SEM.