Comparison of mask file from legacy method and SMASH. (A) Original image of a laminin stained muscle section. (B) Fibers are colored randomly from SMASH output mask with dark regions corresponding to fiber area using a simple threshold. Dark gray areas are interstitial in SMASH and fibers using legacy methods while white areas are interstitial in both masks. Figure demonstrates the larger fiber area obtained with SMASH compared to the legacy method of using a simple threshold for fiber area.