Figure 2

Hepatocyte growth factor-dependent myoblast migration requires glypican-1 expression. (A) Wild-type (WT) C2C12 and C6 myoblasts were seeded onto the upper part of transwell chambers at the same density in serum-free media. The lower chamber contained serum-free media with or without 20 ng/ml hepatocyte growth factor (HGF). After 8 hours, the cells in the upper part of the filter were scraped. The cells that had efficiently migrated through the filter were fixed with paraformaldehyde, stained with crystal violet and photographed or as shown in (B) stained with crystal violet and solubilized in phosphate-buffered saline containing 1% Triton X-100. The absorbance of the detergent soluble fraction at 595 nm was determined. Values are expressed as mean ± standard deviation of three independent experiments. ***P < 0.001 relative to WT control. The migration of WT under control conditions corresponds to a value of 1.0.