Figure 6

miR-206/133b does not modulate abundance of PAX7 in E10.5 embryos. SILAC-based screening of changes in protein abundance based on two independent pairs of miR-206/133b knock-out / wildtype (WT) samples revealed few changes that were greater than 1.5 fold up or down in miR-206/133b knock-out versus WT M. soleus (A). Not one of the proteins expected to be potentially upregulated was a predicted target of miR-206 or miR-133b (microRNA.org). In accordance with transcriptome data, no change in protein abundance could be confirmed in western blot analysis. Loss of miR-206/133b does not change the abundance of PAX7 protein in the trunk of E10.5 embryos (B, C). Analysis of the 3′ untranslated region (UTR) of Pax7 using miRanda indicates identical binding sites for miR-206 and miR-1. Differences in the primary sequence of miR-206 and miR-1 do not lead to substantial changes in the predicted miRNA-Pax7 UTR interaction (D).